Transformation of Riccia fluitans, an Amphibious Liverwort Dynamically Responding to Environmental Changes

Please use this identifier to cite or link to this item:
https://osnadocs.ub.uni-osnabrueck.de/handle/urn:nbn:de:gbv:700-202105114427
Open Access logo originally created by the Public Library of Science (PLoS)
Title: Transformation of Riccia fluitans, an Amphibious Liverwort Dynamically Responding to Environmental Changes
Authors: Althoff, Felix
Zachgo, Sabine
ORCID of the author: https://orcid.org/0000-0002-9132-5833
https://orcid.org/0000-0002-6666-1499
Abstract: The colonization of land by streptophyte algae, ancestors of embryophyte plants, was a fundamental event in the history of life on earth. Bryophytes are early diversifying land plants that mark the transition from freshwater to terrestrial ecosystems. The amphibious liverwort Riccia fluitans can thrive in aquatic and terrestrial environments and thus represents an ideal organism to investigate this major transition. Therefore, we aimed to establish a transformation protocol for R. fluitans to make it amenable for genetic analyses. An Agrobacterium transformation procedure using R. fluitans callus tissue allows to generate stably transformed plants within 10 weeks. Furthermore, for comprehensive studies spanning all life stages, we demonstrate that the switch from vegetative to reproductive development can be induced by both flooding and poor nutrient availability. Interestingly, a single R. fluitans plant can consecutively adapt to different growth environments and forms distinctive and reversible features of the thallus, photosynthetically active tissue that is thus functionally similar to leaves of vascular plants. The morphological plasticity affecting vegetative growth, air pore formation, and rhizoid development realized by one genotype in response to two different environments makes R. fluitans ideal to study the adaptive molecular mechanisms enabling the colonialization of land by aquatic plants.
Citations: Althoff, Felix; Zachgo, Sabine. 2020. "Transformation of Riccia fluitans, an Amphibious Liverwort Dynamically Responding to Environmental Changes" Int. J. Mol. Sci. 21, no. 15: 5410.
URL: https://repositorium.ub.uni-osnabrueck.de/handle/urn:nbn:de:gbv:700-202105114427
Subject Keywords: Riccia fluitans; liverwort; adaptation; terrestrialization; transformation; sexual induction
Issue Date: 29-Jul-2020
License name: Attribution 4.0 International
License url: http://creativecommons.org/licenses/by/4.0/
Type of publication: Einzelbeitrag in einer wissenschaftlichen Zeitschrift [article]
Appears in Collections:FB05 - Hochschulschriften
Open-Access-Publikationsfonds

Files in This Item:
File Description SizeFormat 
ijms_Althoff_Zachgo_2020.pdfArticle4,49 MBAdobe PDF
ijms_Althoff_Zachgo_2020.pdf
Thumbnail
View/Open
ijms_Althoff_Zachgo_Supplement_2020.pdfSupplementary Material359,18 kBAdobe PDF
ijms_Althoff_Zachgo_Supplement_2020.pdf
Thumbnail
View/Open


This item is licensed under a Creative Commons License Creative Commons