Transformation of Riccia fluitans, an Amphibious Liverwort Dynamically Responding to Environmental Changes
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https://osnadocs.ub.uni-osnabrueck.de/handle/urn:nbn:de:gbv:700-202105114427
https://osnadocs.ub.uni-osnabrueck.de/handle/urn:nbn:de:gbv:700-202105114427
Title: | Transformation of Riccia fluitans, an Amphibious Liverwort Dynamically Responding to Environmental Changes |
Authors: | Althoff, Felix Zachgo, Sabine |
ORCID of the author: | https://orcid.org/0000-0002-9132-5833 https://orcid.org/0000-0002-6666-1499 |
Abstract: | The colonization of land by streptophyte algae, ancestors of embryophyte plants, was a fundamental event in the history of life on earth. Bryophytes are early diversifying land plants that mark the transition from freshwater to terrestrial ecosystems. The amphibious liverwort Riccia fluitans can thrive in aquatic and terrestrial environments and thus represents an ideal organism to investigate this major transition. Therefore, we aimed to establish a transformation protocol for R. fluitans to make it amenable for genetic analyses. An Agrobacterium transformation procedure using R. fluitans callus tissue allows to generate stably transformed plants within 10 weeks. Furthermore, for comprehensive studies spanning all life stages, we demonstrate that the switch from vegetative to reproductive development can be induced by both flooding and poor nutrient availability. Interestingly, a single R. fluitans plant can consecutively adapt to different growth environments and forms distinctive and reversible features of the thallus, photosynthetically active tissue that is thus functionally similar to leaves of vascular plants. The morphological plasticity affecting vegetative growth, air pore formation, and rhizoid development realized by one genotype in response to two different environments makes R. fluitans ideal to study the adaptive molecular mechanisms enabling the colonialization of land by aquatic plants. |
Citations: | Althoff, Felix; Zachgo, Sabine. 2020. "Transformation of Riccia fluitans, an Amphibious Liverwort Dynamically Responding to Environmental Changes" Int. J. Mol. Sci. 21, no. 15: 5410. |
URL: | https://osnadocs.ub.uni-osnabrueck.de/handle/urn:nbn:de:gbv:700-202105114427 |
Subject Keywords: | Riccia fluitans; liverwort; adaptation; terrestrialization; transformation; sexual induction |
Issue Date: | 29-Jul-2020 |
License name: | Attribution 4.0 International |
License url: | http://creativecommons.org/licenses/by/4.0/ |
Type of publication: | Einzelbeitrag in einer wissenschaftlichen Zeitschrift [article] |
Appears in Collections: | FB05 - Hochschulschriften Open-Access-Publikationsfonds |
Files in This Item:
File | Description | Size | Format | |
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ijms_Althoff_Zachgo_2020.pdf | Article | 4,49 MB | Adobe PDF | ijms_Althoff_Zachgo_2020.pdf View/Open |
ijms_Althoff_Zachgo_Supplement_2020.pdf | Supplementary Material | 359,18 kB | Adobe PDF | ijms_Althoff_Zachgo_Supplement_2020.pdf View/Open |
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