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Molecular mechanism to target the endosomal Mon1-Ccz1 GEF complex to the pre-autophagosomal structure

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https://osnadocs.ub.uni-osnabrueck.de/handle/urn:nbn:de:gbv:700-201901241066
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dc.creatorJieqiong, Gao-
dc.creatorLangemeyer, Lars-
dc.creatorKümmel, Daniel-
dc.creatorReggiori, Fulvio-
dc.creatorUngermann, Christian-
dc.date.accessioned2019-01-24T08:53:45Z-
dc.date.available2019-01-24T08:53:45Z-
dc.date.issued2018-02-15-
dc.identifier.citationeLife 2018;7:e31145ger
dc.identifier.urihttps://osnadocs.ub.uni-osnabrueck.de/handle/urn:nbn:de:gbv:700-201901241066-
dc.description.abstractDuring autophagy, a newly formed double membrane surrounds its cargo to generate the so-called autophagosome, which then fuses with a lysosome after closure. Previous work implicated that endosomal Rab7/Ypt7 associates to autophagosomes prior to their fusion with lysosomes. Here, we unravel how the Mon1-Ccz1 guanosine exchange factor (GEF) acting upstream of Ypt7 is specifically recruited to the pre-autophagosomal structure under starvation conditions. We find that Mon1-Ccz1 directly binds to Atg8, the yeast homolog of the members of the mammalian LC3 protein family. This requires at least one LIR motif in the Ccz1 C-terminus, which is essential for autophagy but not for endosomal transport. In agreement, only wild-type, but not LIR-mutated Mon1-Ccz1 promotes Atg8-dependent activation of Ypt7. Our data reveal how GEF targeting can specify the fate of a newly formed organelle and provide new insights into the regulation of autophagosome-lysosome fusion.eng
dc.relationhttps://doi.org/10.7554/eLife.31145.001ger
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectendosomal Mon1-Ccz1 GEF complexeng
dc.subjectpre-autophagosomaleng
dc.subject.ddc570 - Biowissenschaften, Biologieger
dc.titleMolecular mechanism to target the endosomal Mon1-Ccz1 GEF complex to the pre-autophagosomal structureeng
dc.typeEinzelbeitrag in einer wissenschaftlichen Zeitschrift [article]ger
orcid.creatorhttps://orcid.org/0000-0003-2652-2686-
dc.identifier.doi10.7554/eLife.31145.001-
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