Kdp-dependent Kplus homeostasis of the halophilic archaeon Halobacterium salinarum
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https://osnadocs.ub.uni-osnabrueck.de/handle/urn:nbn:de:gbv:700-2007121710
https://osnadocs.ub.uni-osnabrueck.de/handle/urn:nbn:de:gbv:700-2007121710
Full metadata record
DC Field | Value | Language |
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dc.contributor.advisor | Prof. Dr. Karlheinz Altendorf | |
dc.creator | Strahl, Henrik | |
dc.date.accessioned | 2010-01-30T14:48:17Z | |
dc.date.available | 2010-01-30T14:48:17Z | |
dc.date.issued | 2007-12-14T13:37:22Z | |
dc.date.submitted | 2007-12-14T13:37:22Z | |
dc.identifier.uri | https://osnadocs.ub.uni-osnabrueck.de/handle/urn:nbn:de:gbv:700-2007121710 | - |
dc.description.abstract | Halobacteria balance high external osmolality by the accumulation of almost equimolar amounts of KCl. Thus, steady Kplus supply is a vital prerequisite for life of these extreme halophiles. So far, Kplus was supposed to enter the cell only passively by use of potential-driven uniporters. However, the genome of the extreme halophilic archaeon Halobacterium sp. NRC-1 comprises one single operon containing the genes kdpFABC coding for homologs of the bacterial ATP-driven Kplus uptake system KdpFABC, together with an additional ORF so far annotated as cat3. Deletion of the kdpFABCcat3 genes led to a reduced ability to grow under limiting Kplus concentrations, whereas real-time RT-PCR measurements revealed both high induction rates and a transcriptional regulation of the Kdp system dependent on external Kplus concentration and growth phase. The synthesis of the high-affinity KdpFABC complex enables H. salinarum to grow under extreme potassium-limiting conditions of down to 20 µM Kplus. These results provide the first experimental evidence of ATP-driven Kplus uptake in halobacteria. The current opinion that Kplus homeostasis of H. salinarum is solely mediated via membrane potential-driven Kplus uniporters is obviously only one aspect of a more complex system. | eng |
dc.language.iso | eng | |
dc.subject | Halobacterium | |
dc.subject | potassium uptake | |
dc.subject | KdpFABC | |
dc.subject | Cat3 | |
dc.subject | universal stress protein | |
dc.subject.ddc | 570 - Biowissenschaften, Biologie | ger |
dc.title | Kdp-dependent Kplus homeostasis of the halophilic archaeon Halobacterium salinarum | eng |
dc.type | Dissertation oder Habilitation [doctoralThesis] | - |
thesis.location | Osnabrück | - |
thesis.institution | Universität | - |
thesis.type | Dissertation [thesis.doctoral] | - |
thesis.date | 2007-11-30T12:00:00Z | - |
elib.elibid | 734 | - |
elib.marc.edt | jost | - |
elib.dct.accessRights | a | - |
elib.dct.created | 2007-12-06T19:00:10Z | - |
elib.dct.modified | 2007-12-14T13:37:22Z | - |
dc.contributor.referee | Prof. Dr. Felicitas Pfeifer | |
dc.subject.bk | 42.13 - Molekularbiologie | ger |
dc.subject.bk | 42.30 - Mikrobiologie | ger |
dc.subject.dnb | 32 - Biologie | ger |
vCard.ORG | FB5 | ger |
Appears in Collections: | FB05 - E-Dissertationen |
Files in This Item:
File | Description | Size | Format | |
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E-Diss734_thesis.pdf | Präsentationsformat | 1,49 MB | Adobe PDF | E-Diss734_thesis.pdf View/Open |
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