Interplay between SERCA, 4E-BP, and eIF4E in the Drosophila heart

Please use this identifier to cite or link to this item:
Open Access logo originally created by the Public Library of Science (PLoS)
Title: Interplay between SERCA, 4E-BP, and eIF4E in the Drosophila heart
Authors: Santalla, Manuela
García, Alejandra
Mattiazzi, Alicia
Valverde, Carlos A.
Schiemann, Ronja
Paululat, Achim
Hernández, Greco
Meyer, Heiko
Ferrero, Paola
ORCID of the author:
Abstract: Appropriate cardiac performance depends on a tightly controlled handling of Ca2+ in a broad range of species, from invertebrates to mammals. The role of the Ca2+ ATPase, SERCA, in Ca2+ handling is pivotal, and its activity is regulated, inter alia, by interacting with distinct proteins. Herein, we give evidence that 4E binding protein (4E-BP) is a novel regulator of SERCA activity in Drosophila melanogaster during cardiac function. Flies over-expressing 4E-BP showed improved cardiac performance in young individuals associated with incremented SERCA activity. Moreover, we demonstrate that SERCA interacts with translation initiation factors eIF4E-1, eIF4E-2 and eIF4E-4 in a yeast two-hybrid assay. The specific identification of eIF4E-4 in cardiac tissue leads us to propose that the interaction of elF4E-4 with SERCA may be the basis of the cardiac effects observed in 4E-BP over-expressing flies associated with incremented SERCA activity.
Citations: Santalla, M., García, A., Mattiazzi, A., Valverde, C. A., Schiemann, R., Paululat, A., Hernández, G., Meyer, H., Ferrero, P. (2022): Interplay between SERCA, 4E-BP, and eIF4E in the Drosophila heart. PLOS ONE, 17(5), e0267156.
Subject Keywords: Drosophila melanogaster; Heart; Hyperexpression techniques; Heart rate; Aging; Cardiovascular physiology; Fluorescence imaging; Protein translation
Issue Date: 19-May-2022
License name: Attribution 4.0 International
License url:
Type of publication: Einzelbeitrag in einer wissenschaftlichen Zeitschrift [Article]
Appears in Collections:FB05 - Hochschulschriften

Files in This Item:
File Description SizeFormat 
Santalla_etal_pone_2022.pdfArticle1,76 MBAdobe PDF

This item is licensed under a Creative Commons License Creative Commons