Morphologically and physiologically diverse fruits of two Lepidium species differ in allocation of glucosinolates into immature and mature seed and pericarp
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https://osnadocs.ub.uni-osnabrueck.de/handle/urn:nbn:de:gbv:700-202105114510
https://osnadocs.ub.uni-osnabrueck.de/handle/urn:nbn:de:gbv:700-202105114510
| Title: | Morphologically and physiologically diverse fruits of two Lepidium species differ in allocation of glucosinolates into immature and mature seed and pericarp |
| Authors: | Mohammed, Said Bhattacharya, Samik Gesing, Matthias Alexander Klupsch, Katharina Theißen, Günther Mummenhoff, Klaus Müller, Caroline |
| ORCID of the author: | https://orcid.org/0000-0001-6151-5217 |
| Abstract: | The morphology and physiology of diaspores play crucial roles in determining the fate of seeds in unpredictable habitats. In some genera of the Brassicaceae different types of diaspores can be found. Lepidium appelianum produces non-dormant seeds within indehiscent fruits while in L. campestre dormant seeds are released from dehiscent fruits. We investigated whether the allocation of relevant defence compounds into different tissues in different Lepidium species may be related to the diverse dispersal strategy (indehiscent and dehiscent) and seed physiology (non-dormant and dormant). Total glucosinolate concentration and composition were analysed in immature and mature seeds and pericarps of L. appelianum and L. campestre using high-performance liquid chromatography. Moreover, for comparison, transgenic RNAi L. campestre lines were used that produce indehiscent fruits due to silencing of LcINDEHISCENCE, the INDEHISCENCE ortholog of L. campestre. Total glucosinolate concentrations were lower in immature compared to mature seeds in all studied Lepidium species and transgenic lines. In contrast, indehiscent fruits of L. appelianum maintained their total glucosinolate concentration in mature pericarps compared to immature ones, while in dehiscent L. campestre and in indehiscent RNAi-LcIND L. campestre a significant decrease in total glucosinolate concentrations from immature to mature pericarps could be detected. Indole glucosinolates were detected in lower abundance than the other glucosinolate classes (aliphatic and aromatic). Relatively high concentrations of 4-methoxyindol-3-ylmethyl glucosinolate were found in mature seeds of L. appelianum compared to other tissues, while no indole glucosinolates were detected in mature diaspores of L. campestre. The diaspores of the latter species may rather depend on aliphatic and aromatic glucosinolates for long-term protection. The allocation patterns of glucosinolates correlate with the morpho-physiologically distinct fruits of L. appelianum and L. campestre and may be explained by the distinct dispersal strategies and the dormancy status of both species. |
| Citations: | Mohammed S, Bhattacharya S, Gesing MA, Klupsch K, Theißen G, et al. (2020) Morphologically and physiologically diverse fruits of two Lepidium species differ in allocation of glucosinolates into immature and mature seed and pericarp. PLOS ONE 15(8): e0227528 |
| URL: | https://osnadocs.ub.uni-osnabrueck.de/handle/urn:nbn:de:gbv:700-202105114510 |
| Subject Keywords: | Seeds; Pericarp; Fruits; Morphogenesis; Indoles; Genetically modified plants; Plant defenses; Seed germination |
| Issue Date: | 25-Aug-2020 |
| License name: | Attribution 4.0 International |
| License url: | http://creativecommons.org/licenses/by/4.0/ |
| Type of publication: | Einzelbeitrag in einer wissenschaftlichen Zeitschrift [article] |
| Appears in Collections: | FB05 - Hochschulschriften Open-Access-Publikationsfonds |
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| File | Description | Size | Format | |
|---|---|---|---|---|
| Bhattacharya_Article_pone_0227528_2020.pdf | Article | 2,39 MB | Adobe PDF | Bhattacharya_Article_pone_0227528_2020.pdf  View/Open |
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