Title:	Comprehensive Single Cell Analyses of the Nutritional Environment of Intracellular Salmonella enterica
Authors:	Röder, Jennifer Felgner, Pascal Michael, Hensel
ORCID of the author:	https://orcid.org/0000-0001-6604-6253 https://orcid.org/0000-0002-1682-6873 https://orcid.org/0000-0001-7554-211X
Abstract:	The facultative intracellular pathogen Salmonella enterica Typhimurium (STM) resides in a specific membrane-bound compartment termed the Salmonella-containing vacuole (SCV). STM is able to obtain all nutrients required for rapid proliferation, although being separated from direct access to host cell metabolites. The formation of specific tubular membrane compartments, called Salmonella-induced filaments (SIFs) are known to provides bacterial nutrition by giving STM access to endocytosed material and enabling proliferation. Additionally, STM expresses a range of nutrient uptake system for growth in nutrient limited environments to overcome the nutrition depletion inside the host. By utilizing dual fluorescence reporters, we shed light on the nutritional environment of intracellular STM in various host cells and distinct intracellular niches. We showed that STM uses nutrients of the host cell and adapts uniquely to the different nutrient conditions. In addition, we provide further evidence for improved nutrient supply by SIF formation or presence in the cytosol of epithelial cells, and the correlation of nutrient supply to bacterial proliferation.
Citations:	Röder J, Felgner P, Hensel M.: Comprehensive Single Cell Analyses of the Nutritional Environment of Intracellular Salmonella enterica. Front Cell Infect Microbiol. 2021 Mar 23;11:624650. doi: 10.3389/fcimb.2021.624650. PMID: 33834004; PMCID: PMC8021861.
URL:	https://osnadocs.ub.uni-osnabrueck.de/handle/urn:nbn:de:gbv:700-202105064363
Subject Keywords:	Salmonella-containing vacuole; flow cytometry; intracellular pathogen; nutritional limitation; single cell analyses
Issue Date:	23-Mar-2021
License name:	Attribution 4.0 International
License url:	http://creativecommons.org/licenses/by/4.0/
Type of publication:	Einzelbeitrag in einer wissenschaftlichen Zeitschrift [article]
Appears in Collections:	FB05 - Hochschulschriften Open-Access-Publikationsfonds

This item is licensed under a Creative Commons License