Signal transduction and oligomerization – the role of a phototransducer signaling domain

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dc.contributor.advisorProf. Dr. Heinz-Jürgen Steinhoffger
dc.creatorOrban-Glaß, Ioan-
dc.description.abstractThe signal transduction pathway of halophilic archaea remains a fascinating example of adaptation to extreme environments. Despite similarities with bacterial taxis systems, its structural and dynamics patterns during signal relay remain debatable. The currently investigated SRII/HtrII phototaxis system of Natronomonas pharaonis shows remarkable similarities with chemoreceptors in its membrane and HAMP domains functioning design. By combining site-directed spin labeling (SDSL) with electron paramagnetic resonance (EPR) spectroscopy we investigate the kinase control domain (i.e. signaling domain) of NpSRII/HtrII both in terms of dynamic and structural properties. Our data, as provided by continuous wave and pulse (DEER) EPR techniques, builds on current dynamics based signaling models for HAMP domains (such as the “frozen–dynamic” or two-state equilibrium models). We present an expanded mechanism for signal propagation throughout the signaling domain, where salt and temperature variations trigger subtle shifts in dynamics. Extreme dynamics motional ranges (compact or highly-dynamic) associate with a specific flagellar signaling state, here the kinase-off response, where a more moderate dynamics motion (dynamic) associates with the kinase-on response. Structurally, we reference our data on PML and ND reconstituted NpSRII/HtrII to the EcTsr crystal structure and the NpHtrII homology model. We show that, despite a difference in packing, NpHtrII oligomerizes in a similar manner as EcTsr, even in the absence of stabilizing structures such as the CheA/CheW baseplate. The presence of trimers-of-dimers but also dimers-of-dimers in membrane sheet samples exposes the high affinity with which NpHtrII signaling domains interact. We hope our structural and dynamics details will push further not just drug design but also environmental preservation efforts where taxis systems drive colonization and virulence of pathogens in plants, animals and humans alike.eng
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Germany*
dc.subjectElectron Paramagnetic Resonanceeng
dc.subjectsignaling domaineng
dc.subjecttwo-component pathwayeng
dc.subject.ddc530 - Physikger
dc.subject.ddc570 - Biowissenschaften, Biologieger
dc.titleSignal transduction and oligomerization – the role of a phototransducer signaling domaineng
dc.typeDissertation oder Habilitation [doctoralThesis]-
thesis.typeDissertation [thesis.doctoral]-
dc.contributor.refereeProf. Dr. Wolfgang Harneitger
Appears in Collections:FB04 - E-Dissertationen

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