1. osnaDocs
  2. Fachbereich 06 - Mathematik / Informatik / Physik
  3. FB06 - E-Dissertationen

Time-resolved and temperature-dependent EPR of conformational dynamics: A spectroscopic view of IGPS, SRII/HtrII and ChR2

Please use this identifier to cite or link to this item:
https://osnadocs.ub.uni-osnabrueck.de/handle/urn:nbn:de:gbv:700-202006103090
Open Access logo originally created by the Public Library of Science (PLoS)
Full metadata record
DC FieldValueLanguage
dc.contributor.advisorProf. Dr. Heinz-Jürgen Steinhoffger
dc.creatorSchumacher, Magdalena-
dc.date.accessioned2020-06-10T10:08:24Z-
dc.date.available2020-06-10T10:08:24Z-
dc.date.issued2020-06-10T10:08:25Z-
dc.identifier.urihttps://osnadocs.ub.uni-osnabrueck.de/handle/urn:nbn:de:gbv:700-202006103090-
dc.description.abstractIn the present work three different proteins were investigated, namely IGPS, the SRII/HtrII complex and ChR2. EPR spectroscopy combined with SDSL were applied to investigate the relation of the respective protein dynamics and their functions. In case of IGPS spin label side chain dynamics were recorded and allow a classification of loop dynamics during catalysis. For SRII/HtrII an equilibrium of spin label side chain dynamics was found to reflect the equilibrium of the activated and deactivated states, which are responsible for signal transfer through the complex. Time-resolved analyses of side chain dynamics and trapping of intermediates of the light-triggered channel ChR2 identified conformational changes to occur during channel function. Thereby, the EPR spectroscopy proves to be a powerful tool to investigate proteins in context of structural changes during their protein function.eng
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Germany*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/de/*
dc.subjectElectron paramagnetic resonanceeng
dc.subjectElectron spin resonanceeng
dc.subject.ddc530 - Physikger
dc.titleTime-resolved and temperature-dependent EPR of conformational dynamics: A spectroscopic view of IGPS, SRII/HtrII and ChR2eng
dc.typeDissertation oder Habilitation [doctoralThesis]-
thesis.locationOsnabrück-
thesis.institutionUniversität-
thesis.typeDissertation [thesis.doctoral]-
thesis.date2020-03-13-
dc.contributor.refereeProf. Dr. Daniel Kümmelger
Appears in Collections:FB06 - E-Dissertationen

Files in This Item:
File Description SizeFormat 
thesis_schumacher.pdfPräsentationsformat45,78 MBAdobe PDF
thesis_schumacher.pdf
Thumbnail
View/Open
Show simple item record


This item is licensed under a Creative Commons License Creative Commons