Time-resolved and temperature-dependent EPR of conformational dynamics: A spectroscopic view of IGPS, SRII/HtrII and ChR2

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Titel: Time-resolved and temperature-dependent EPR of conformational dynamics: A spectroscopic view of IGPS, SRII/HtrII and ChR2
Autor(en): Schumacher, Magdalena
Erstgutachter: Prof. Dr. Heinz-Jürgen Steinhoff
Zweitgutachter: Prof. Dr. Daniel Kümmel
Zusammenfassung: In the present work three different proteins were investigated, namely IGPS, the SRII/HtrII complex and ChR2. EPR spectroscopy combined with SDSL were applied to investigate the relation of the respective protein dynamics and their functions. In case of IGPS spin label side chain dynamics were recorded and allow a classification of loop dynamics during catalysis. For SRII/HtrII an equilibrium of spin label side chain dynamics was found to reflect the equilibrium of the activated and deactivated states, which are responsible for signal transfer through the complex. Time-resolved analyses of side chain dynamics and trapping of intermediates of the light-triggered channel ChR2 identified conformational changes to occur during channel function. Thereby, the EPR spectroscopy proves to be a powerful tool to investigate proteins in context of structural changes during their protein function.
URL: https://repositorium.ub.uni-osnabrueck.de/handle/urn:nbn:de:gbv:700-202006103090
Schlagworte: Electron paramagnetic resonance; Electron spin resonance
Erscheinungsdatum: 10-Jun-2020
Lizenzbezeichnung: Attribution-NonCommercial-NoDerivs 3.0 Germany
URL der Lizenz: http://creativecommons.org/licenses/by-nc-nd/3.0/de/
Publikationstyp: Dissertation oder Habilitation [doctoralThesis]
Enthalten in den Sammlungen:FB04 - E-Dissertationen

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