Title:	Functional expression of the entire adhesiome of Salmonella enterica serotype Typhimurium
Authors:	Hansmeier, Nicole Miskiewicz, Katarzyna Elpers, Laura Liss, Viktoria Hensel, Michael Sterzenbach, Torsten
Abstract:	Adhesins are crucial virulence factors of pathogenic bacteria involved in colonization, transmission and pathogenesis. Many bacterial genomes contain the information for a surprisingly large number of diverse adhesive structures. One prominent example is the invasive and facultative intracellular pathogen Salmonella enterica with an adhesiome of up to 20 adhesins. Such large repertoire of adhesins contributes to colonization of a broad range of host species and may allow adaptation to various environments within the host, as well as in non-host environments. For S . enterica , only few members of the adhesiome are functionally expressed under laboratory conditions, and accordingly the structural and functional understanding of the majority of adhesins is sparse. We have devised a simple and versatile approach to functionally express all adhesins of S . enterica serotype Typhimurium, either within Salmonella or within heterologous hosts such as Escherichia coli . We demonstrate the surface expression of various so far cryptic adhesins and show ultrastructural features using atomic force microscopy and transmission electron microscopy. In summary, we report for the first time the expression of the entire adhesiome of S . enterica serotype Typhimurium.
Citations:	Scientific Reports 7:10326 (2017) Springer Nature
URL:	https://osnadocs.ub.uni-osnabrueck.de/handle/urn:nbn:de:gbv:700-2018050217048
Subject Keywords:	Adhesins; Salmonella enterica typhimurium; expression
Issue Date:	2-May-2018
License name:	Namensnennung 4.0 International
License url:	http://creativecommons.org/licenses/by/4.0/
Type of publication:	Einzelbeitrag in einer wissenschaftlichen Zeitschrift [article]
Appears in Collections:	FB05 - Hochschulschriften Open-Access-Publikationsfonds

This item is licensed under a Creative Commons License