Functional expression of the entire adhesiome of Salmonella enterica serotype Typhimurium

Bitte benutzen Sie diese Kennung, um auf die Ressource zu verweisen:
Open Access logo originally created by the Public Library of Science (PLoS)
Titel: Functional expression of the entire adhesiome of Salmonella enterica serotype Typhimurium
Autor(en): Hansmeier, Nicole
Miskiewicz, Katarzyna
Elpers, Laura
Liss, Viktoria
Hensel, Michael
Sterzenbach, Torsten
Zusammenfassung: Adhesins are crucial virulence factors of pathogenic bacteria involved in colonization, transmission and pathogenesis. Many bacterial genomes contain the information for a surprisingly large number of diverse adhesive structures. One prominent example is the invasive and facultative intracellular pathogen Salmonella enterica with an adhesiome of up to 20 adhesins. Such large repertoire of adhesins contributes to colonization of a broad range of host species and may allow adaptation to various environments within the host, as well as in non-host environments. For S . enterica , only few members of the adhesiome are functionally expressed under laboratory conditions, and accordingly the structural and functional understanding of the majority of adhesins is sparse. We have devised a simple and versatile approach to functionally express all adhesins of S . enterica serotype Typhimurium, either within Salmonella or within heterologous hosts such as Escherichia coli . We demonstrate the surface expression of various so far cryptic adhesins and show ultrastructural features using atomic force microscopy and transmission electron microscopy. In summary, we report for the first time the expression of the entire adhesiome of S . enterica serotype Typhimurium.
Bibliografische Angaben: Scientific Reports 7:10326 (2017) Springer Nature
Schlagworte: Adhesins; Salmonella enterica typhimurium; expression
Erscheinungsdatum: 2-Mai-2018
Lizenzbezeichnung: Namensnennung 4.0 International
URL der Lizenz:
Publikationstyp: Einzelbeitrag in einer wissenschaftlichen Zeitschrift [article]
Enthalten in den Sammlungen:FB05 - Hochschulschriften

Dateien zu dieser Ressource:
Datei Beschreibung GrößeFormat 
Scientific_Reports_7_10326_2017_Sterzenbach.pdf3,11 MBAdobe PDF

Diese Ressource wurde unter folgender Copyright-Bestimmung veröffentlicht: Lizenz von Creative Commons Creative Commons