Architecture and mechanism of the late endosomal Rab7-like Ypt7 guanine nucleotide exchange factor complex Mon1–Ccz1

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dc.creatorKiontke, Stephan-
dc.creatorLangemeyer, Lars-
dc.creatorKuhlee, Anne-
dc.creatorSchuback, Saskia-
dc.creatorRaunser, Stefan-
dc.creatorUngermann, Christian-
dc.creatorKümmel, Daniel-
dc.date.accessioned2018-04-30T08:47:55Z-
dc.date.available2018-04-30T08:47:55Z-
dc.date.issued2018-04-30T08:47:55Z-
dc.identifier.citationNature Communications 8, Article number: 14034, 2017-
dc.identifier.urihttps://osnadocs.ub.uni-osnabrueck.de/handle/urn:nbn:de:gbv:700-2018043016920-
dc.description.abstractThe Mon1–Ccz1 complex (MC1) is the guanine nucleotide exchange factor (GEF) for the Rab GTPase Ypt7/Rab7 and is required for endosomal maturation and fusion at the vacuole/ lysosome. Here we present the overall architecture of MC1 from Chaetomium thermophilum , and in combining biochemical studies and mutational analysis in yeast, we identify the domains required for catalytic activity, complex assembly and localization of MC1. The crystal structure of a catalytic MC1 core complex bound to Ypt7 provides mechanistic insight into its function. We pinpoint the determinants that allow for a discrimination of the Rab7-like Ypt7 over the Rab5-like Vps21, which are both located on the same membrane. MC1 shares structural similarities with the TRAPP complex, but employs a novel mechanism to promote nucleotide exchange that utilizes a conserved lysine residue of Ypt7, which is inserted upon MC1 binding into the nucleotide-binding pocket of Ypt7 and contributes to specificity.eng
dc.relationhttps://www.nature.com/articles/ncomms14034.pdf-
dc.rightsNamensnennung 4.0 International-
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/-
dc.subjectEndosomeeng
dc.subjectMC1und
dc.subjectGuanine nucleotide exchange factoreng
dc.subjectRab GTPaseund
dc.subjectYpt7und
dc.subjectRab7und
dc.subject.ddc570 - Biowissenschaften, Biologie-
dc.titleArchitecture and mechanism of the late endosomal Rab7-like Ypt7 guanine nucleotide exchange factor complex Mon1–Ccz1eng
dc.typeEinzelbeitrag in einer wissenschaftlichen Zeitschrift [article]ger
dc.identifier.doi10.1038/ncomms14034-
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