Architecture and mechanism of the late endosomal Rab7-like Ypt7 guanine nucleotide exchange factor complex Mon1–Ccz1
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https://osnadocs.ub.uni-osnabrueck.de/handle/urn:nbn:de:gbv:700-2018043016920
https://osnadocs.ub.uni-osnabrueck.de/handle/urn:nbn:de:gbv:700-2018043016920
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DC Element | Wert | Sprache |
---|---|---|
dc.creator | Kiontke, Stephan | - |
dc.creator | Langemeyer, Lars | - |
dc.creator | Kuhlee, Anne | - |
dc.creator | Schuback, Saskia | - |
dc.creator | Raunser, Stefan | - |
dc.creator | Ungermann, Christian | - |
dc.creator | Kümmel, Daniel | - |
dc.date.accessioned | 2018-04-30T08:47:55Z | - |
dc.date.available | 2018-04-30T08:47:55Z | - |
dc.date.issued | 2018-04-30T08:47:55Z | - |
dc.identifier.citation | Nature Communications 8, Article number: 14034, 2017 | - |
dc.identifier.uri | https://osnadocs.ub.uni-osnabrueck.de/handle/urn:nbn:de:gbv:700-2018043016920 | - |
dc.description.abstract | The Mon1–Ccz1 complex (MC1) is the guanine nucleotide exchange factor (GEF) for the Rab GTPase Ypt7/Rab7 and is required for endosomal maturation and fusion at the vacuole/ lysosome. Here we present the overall architecture of MC1 from Chaetomium thermophilum , and in combining biochemical studies and mutational analysis in yeast, we identify the domains required for catalytic activity, complex assembly and localization of MC1. The crystal structure of a catalytic MC1 core complex bound to Ypt7 provides mechanistic insight into its function. We pinpoint the determinants that allow for a discrimination of the Rab7-like Ypt7 over the Rab5-like Vps21, which are both located on the same membrane. MC1 shares structural similarities with the TRAPP complex, but employs a novel mechanism to promote nucleotide exchange that utilizes a conserved lysine residue of Ypt7, which is inserted upon MC1 binding into the nucleotide-binding pocket of Ypt7 and contributes to specificity. | eng |
dc.relation | https://www.nature.com/articles/ncomms14034.pdf | - |
dc.rights | Namensnennung 4.0 International | - |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | - |
dc.subject | Endosome | eng |
dc.subject | MC1 | und |
dc.subject | Guanine nucleotide exchange factor | eng |
dc.subject | Rab GTPase | und |
dc.subject | Ypt7 | und |
dc.subject | Rab7 | und |
dc.subject.ddc | 570 - Biowissenschaften, Biologie | - |
dc.title | Architecture and mechanism of the late endosomal Rab7-like Ypt7 guanine nucleotide exchange factor complex Mon1–Ccz1 | eng |
dc.type | Einzelbeitrag in einer wissenschaftlichen Zeitschrift [article] | ger |
dc.identifier.doi | 10.1038/ncomms14034 | - |
vCard.ORG | FB5 | - |
Enthalten in den Sammlungen: | FB05 - Hochschulschriften Open-Access-Publikationsfonds |
Dateien zu dieser Ressource:
Datei | Beschreibung | Größe | Format | |
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ncomms_8_14034_2017_Kuemmel.pdf | 1,48 MB | Adobe PDF | ncomms_8_14034_2017_Kuemmel.pdf Öffnen/Anzeigen |
Diese Ressource wurde unter folgender Copyright-Bestimmung veröffentlicht: Lizenz von Creative Commons