Photopatterning for probing protein-protein interactions in artificial model systems and live cells
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https://osnadocs.ub.uni-osnabrueck.de/handle/urn:nbn:de:gbv:700-2012101510347
https://osnadocs.ub.uni-osnabrueck.de/handle/urn:nbn:de:gbv:700-2012101510347
Title: | Photopatterning for probing protein-protein interactions in artificial model systems and live cells |
Authors: | Waichman, Sharon |
Thesis advisor: | Prof. Dr. Jacob Piehler |
Thesis referee: | Prof. Dr. Heinz-Jürgen Steinhoff |
Abstract: | Functional immobilization and lateral organization of proteins into micro- and nanopatterns is an important prerequisite for miniaturizing analytical and biotechnological devices. In this thesis I present novel and versatile approaches for high contrast surface micropatterning of proteins, artificial membranes and live cells based on maleimide photochemistry. The patterning strategy is carried-out on glass substrates exploiting a poly(ethylene glycol) PEG polymer layer as a compatible scaffold. The flexible PEG cushion prevents unspecific proteins attachment and cell adhesion to surfaces. The versatility of this method is demonstrated by means of different orthogonal chemistries using covalent- and affinity- based interactions for protein immobilization. Furthermore, using maleimide based alkyl-thiol chemistry, I utilized the patterning approach for capturing liposomes and proteoliposomes onto surfaces. Formation of fluid patterned polymer-supported membranes demonstrating lateral diffusion of lipids and proteins was confirmed by biophysical assays. A similar approach was used for micropatterning of transmembrane proteins in surface adhered live cells. |
URL: | https://osnadocs.ub.uni-osnabrueck.de/handle/urn:nbn:de:gbv:700-2012101510347 |
Subject Keywords: | Surface chemistry; Protein-protein interactions; Photopatterning |
Issue Date: | 15-Oct-2012 |
Type of publication: | Dissertation oder Habilitation [doctoralThesis] |
Appears in Collections: | FB05 - E-Dissertationen |
Files in This Item:
File | Description | Size | Format | |
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thesis_waichman.pdf | Präsentationsformat | 7,42 MB | Adobe PDF | thesis_waichman.pdf View/Open |
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