Titel:	The protected physiological state of intracellular Salmonella enterica persisters reduces host cell-imposed stress
Autor(en):	Schulte, Marc Olschewski, Katharina Hensel, Michael
ORCID des Autors:	https://orcid.org/0000-0001-6604-6253 https://orcid.org/0000-0002-2371-8272 https://orcid.org/0000-0003-4308-2002
Zusammenfassung:	During infectious diseases, small subpopulations of bacterial pathogens enter a non-replicating (NR) state tolerant to antibiotics. After phagocytosis, intracellular Salmonella enterica serovar Typhimurium (STM) forms persisters able to subvert immune defenses of the host. Physiological state and sensing properties of persisters are difficult to analyze, thus poorly understood. Here we deploy fluorescent protein reporters to detect intracellular NR persister cells, and to monitor their stress response on single cell level. We determined metabolic properties of NR STM during infection and demonstrate that NR STM persisters sense their environment and respond to stressors. Since persisters showed a lower stress response compared to replicating (R) STM, which was not consequence of lower metabolic capacity, the persistent state of STM serves as protective niche. Up to 95% of NR STM were metabolically active at beginning of infection, very similar to metabolic capacity of R STM. Sensing and reacting to stress with constant metabolic activity supports STM to create a more permissive environment for recurrent infections. Stress sensing and response of persister may be targeted by new antimicrobial approaches.
Bibliografische Angaben:	Schulte, M., Olschewski, K. & Hensel, M.: The protected physiological state of intracellular Salmonella enterica persisters reduces host cell-imposed stress. Commun Biol 4, 520 (2021).
URL:	https://doi.org/10.48693/46 https://osnadocs.ub.uni-osnabrueck.de/handle/ds-202202086133
Schlagworte:	Bacteriology; Pathogens
Erscheinungsdatum:	4-Mai-2021
Lizenzbezeichnung:	Attribution 4.0 International
URL der Lizenz:	http://creativecommons.org/licenses/by/4.0/
Publikationstyp:	Einzelbeitrag in einer wissenschaftlichen Zeitschrift [article]
Enthalten in den Sammlungen:	FB05 - Hochschulschriften Open-Access-Publikationsfonds

Diese Ressource wurde unter folgender Copyright-Bestimmung veröffentlicht: Lizenz von Creative Commons